Our lab is interested in unraveling the mechanisms governing growth and differentiation in the developing nervous system. Our current focus is the identification of cell contact- as well as diffusible factor-mediated determinants of neural lineage specification.
We utilize human pluripotent stem cells (embryonic stem cells and induced pluripotent stem cells) as the chief model system, complemented with primary neural cell cultures, tumor cell lines and transgenic mouse model systems. Important techniques include a number of cell and tissue culture-based assays, molecular and cell biology, flow cytometry and FACS, histological analysis and microscopy.
Please see also: http://pruszak.openwetware.org/
D.C. Schöndorf, M. Aureli, F.E. McAllister, C.J. Hindley, F. Mayer, B. Schmid, S.P. Sardi, M. Valsecchi, S. Hoffmann, L.K. Schwarz, U. Hedrich, D. Berg, L.S. Shihabuddin, J. Hu, J.Pruszak, S.P. Gygi, S. Sonnino, T. Gasser, M. Deleidi: iPSC-derived neurons from GBA1-associated Parkinson’s disease patients show autophagic defects and impaired calcium homeostasis (2014). Nature Communications, 5: 4028.
G. Turaç, C.J. Hindley, R. Thomas, J.A. Davis, M. Deleidi, T. Gasser, E. Karaöz, J.Pruszak (2013) Combined flow cytometric analysis of surface and intracellular antigens reveals surface molecule markers of human neuropoiesis. PLOS ONE , 8(6): e68519.
Schlegelmilch K., Mohseni M., Kirak O., Pruszak J., Rodriguez JR., Zhou D., Kreger BT., Vasioukhin V., Avruch J., Brummelkamp TR., Camargo FD. (2011) Yap1 acts downstream of α-catenin to control epidermal proliferation. J Cell Sci., 144: 782-95.
Carette* JE*., Pruszak J.*, Varadarajan M., Blomen VA., Gokhale S., Camargo FD., Wernig M., Jaenisch R., Brummelkamp TR. (2010) Generation of iPSCs from cultured human malignant cells. Blood, 115: 4039-42.
Chung S., Leung A., Han BS., Chang MY., Moon JI., Kim CH., Hong S., Pruszak J., Isacson O., Kim KS. (2009) Wnt1-lmx1a forms a novel autoregulatory loop and controls midbrain dopaminergic differentiation synergistically with the SHH-FoxA2 pathway. Cell Stem Cell, 5: 646-58.
2012 Editorial Board Memberships: PLoS ONE, Molecular and Cellular Therapies (and 2013)
2011 Group leader, Emmy Noether-Program of the DFG
2009 Morgan-Zinsser Fellowship, The Academy at Harvard Medical School
2009 AAAS/ Science Program for Excellence in Science
2009 Co-recipient, Cozzarelli Prize, National Academy of Sciences (for Wernig et al., 2008)
2009 Excellence in Tutoring Award, Harvard Medical School (and 2010)
2007 Grave Scholarship, Shifman Endowed Scholarship, Marine Biological Laboratory (MBL)
2007 Nominee, Harvard Medical School Faculty Prize for Excellence in Teaching