a tissue-slice preparation was developed to study single-cell physiology, secretory function and development of neurosecretory cells, such as the beta cells of the pancreas and pituitary cells. One of the aims of the group is a better understanding of differentiation processes, which eventually may
lead to advances in cellular replacement therapy.

http://www.mf.uni-mb.si/fizio/rupnik.html

Yang S-B, Major F, Tietze LT, Rupnik M(2005):Block of delayed-rectifier potassium channels by reduced-haloperidol and related compounds in mouse cortical neurons..(2005) J Pharmacol Exp Ther. 315(1):352-362

Sedej S, Rose T, Rupnik M(2005):cAMP increases Ca2+-dependent exocytosis through both PKA and Epac2 in mouse melanotrophs from pituitary tissue slices..J Physiol. 567(3):799-813

Rupnik M, Pabst S, Rupnik M, von Eichel-Streiber C, Urlaub H, Soling HD(2005):Characterization of the cleavage site and function of resulting cleavage fragments after limited proteolysis of Clostridium difficile toxin B (TcdB) by host cells..Microbiology. 151(Pt 1):199-208

Turner J-E, Sedej S, Rupnik M(2005):Cytosolic Cl- ions in the regulation of secretory and endocytotic activity in melanotrophs from mouse pituitary tissue slices...J Physiol. 566(2):443-453

Blyszczuk P, Asbrand C, Rozzo A, Kania G, St-Onge L, Rupnik M, Wobus AM(2005):Embryonic stem cells differentiate into insulin-producing cells without selection of nestin-expressing cells..Int J Dev Biol. 48(10):1095-104

Sedej, S., Rupnik, M., Zorec, R.(2005):Endocytosis requires Rab5 protein in rat melanotrophs..(2005) Ann NY Acad Sci 1948:272-280

Speier S, Yang S-B, Sroka K, Rose T, Rupnik M.(2005):KATP channels in beta-cells in pancreatic slices are directly modulated by millimolar ATP..Mol Cell Endocrinol. 230(1-2):51-8

Petrovi? U, Šribar J, Pariš A, Rupnik M, Limpel-Kržan M, Vardjan N, Gubenšek F, Zorec R, Križaj I(2004):Ammodytoxin, a neurotoxic secretory phospholipase A2, can act in the cytosol of the nerve cell..Biochem Biophys Res Comm, 324:981-985

Cocucci E, Racchetti G, Podini P, Rupnik M, Meldolesi J.(2004):Enlargeosome, an Exocytic Vesicle Resistant to Non-Ionic Detergents, Undergoes Endocytosis via a Non-Acidic Route..Mol Biol Cell. 15(12):5356-68

Meneghel-Rozzo, T., Rozzo, A., Poppi, L., Rupnik, M.(2004):In vivo and in vitro development of mouse pancreatic ?-cells in the organotypic slices..Cell Tiss Res, 316:295-303

Yang S-B, Proks P, Ashcroft F M, Rupnik M.(2004):Inhibition of ATP-sensitive potassium channels by haloperidol..Br J Pharmacol. 143(8):981-985

Kreft, M., Stenovec, M., Rupnik, M., et al..(2004):Properties of Ca2+-dependent exocytosis in cultured astrocytes..Glia, 46 (4):437-445

Sedej S, Tsujimoto T, Zorec R, Rupnik M.(2004):Voltage-activated Ca2+ channels and their role in the endocrine function of the pituitary gland in newborn and adult mice..J Physiol:

Speier, S, Rupnik, M.(2003):A novel approach to in situ characterization of pancreatic b-cells..Pflugers Arch-Eur J Physiol 446(5):553-558